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元件指南

VectorBuilder提供了許多常用的載體元件，可供用戶在設計載體時進行選擇。下表按類型提供了這些常用元件的詳細信息。

CAR-T相關(guān)結(jié)構(gòu)域

姓名	描述	Application Notes	Length	Details
CD8-leader	Leader signal peptide of T-cell surface glycoprotein CD8 alpha chain	Directs transport and localization of the protein to the T-cell surface.	60 bp	View
CD8-hinge	Hinge region of T-cell surface glycoprotein CD8 alpha chain	Connects scFv and the transmembrane region; enhances tumor recognition efficiency; improves the expansion of CAR-T cells.	144 bp	View
CD28-hinge	Hinge region of the T-cell surface glycoprotein CD28	Connects scFv with the transmembrane region providing stability and flexibility for efficient CAR expression and function; enhances efficiency of tumor recognition; improves expansion of CAR-T cells.	117 bp	View
CD8-TM	Transmembrane region of T-cell surface glycoprotein CD8 alpha chain	Anchors the CAR to the plasma membrane and bridges the extracellular hinge as well as antigen recognition domains with the intracellular signaling region; enhances receptor expression and stability.	72 bp	View
CD28-TM	Transmembrane region of T-cell-surface glycoprotein CD28	Anchors the CAR to the plasma membrane and bridges the extracellular hinge as well as antigen recognition domains with the intracellular signaling region; enhances receptor expression and stability.	81 bp	View

激活結(jié)構(gòu)域

姓名	描述	Application Notes	Length	Details
CD3zeta	Intracellular domain of the T cell receptor-CD3ζ chain	Acts as a stimulatory molecule for activating T cell-mediated immune response.	336 bp	View

共刺激結(jié)構(gòu)域

姓名	描述	Application Notes	Length	Details
CD28	CD28 intracellular costimulatory domain	Improves overall survival, proliferation and persistence of activated CAR-T cells.	123 bp	View
4-1BB	4-1BB intracellular costimulatory domain	Improves overall survival, proliferation and persistence of activated CAR-T cells; further enhances cytotoxicity and durability when used in combination with CD28.	126 bp	View

scFv

姓名	描述	Application Notes	Length	Details
CD19-scFv	Single-chain variable fragment derived from CD19 antibody	Recognizes human CD19-expressing cells in an antigen-specific manner.	744 bp	View

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