服務(wù)商工作站 >>
元件指南
VectorBuilder提供了許多常用的載體元件，可供用戶在設(shè)計(jì)載體時(shí)進(jìn)行選擇。下表按類型提供了這些常用元件的詳細(xì)信息。
備注:
連接子（linker）用于連接由單個(gè)啟動(dòng)子驅(qū)動(dòng)的多個(gè)ORF同時(shí)表達(dá)。連接子（linker）有兩種類型：
連接子（linker）用于連接由單個(gè)啟動(dòng)子驅(qū)動(dòng)的多個(gè)ORF同時(shí)表達(dá)。連接子（linker）有兩種類型：
- 通讀型連接子（例如融合連接子或2A肽）：上游ORF缺少終止密碼子，使得翻譯從上游ORF通過(guò)連接子進(jìn)入下游的ORF。
- 非通讀型連接子（例如IRES）：上游ORF以終止密碼子結(jié)束，使得上游ORF的翻譯在到達(dá)接頭之前終止。
融合蛋白（通讀型連接子）
2A肽（通讀型連接子）
| 姓名 | 描述 | Application Notes | Length | Details |
|---|---|---|---|---|
| T2A | Self-cleaving 2A peptide from Thosea asigna virus | Causes co-translational cleavage of the encoded polypeptide. Multiple proteins can be made from a polycistronic transcript containing multiple ORFs separated by 2A. P2A and T2A have higher cleavage efficiency compared to other 2As. | 63 bp | View |
| P2A | Self-cleaving 2A peptide from Porcine teschovirus-1 | Causes co-translational cleavage of the encoded polypeptide. Multiple proteins can be made from a polycistronic transcript containing multiple ORFs separated by 2A. P2A and T2A have higher cleavage efficiency compared to other 2As. | 66 bp | View |
| F2A | Self-cleaving 2A peptide from foot-and-mouth disease virus | Causes co-translational cleavage of the encoded polypeptide. Multiple proteins can be made from a polycistronic transcript containing multiple ORFs separated by 2A. | 75 bp | View |
| E2A | Self-cleaving 2A peptide from Equine rhinitis A virus | Causes co-translational cleavage of the encoded polypeptide. Multiple proteins can be made from a polycistronic transcript containing multiple ORFs separated by 2A. | 69 bp | View |
| T2A(dme) | Self-cleaving 2A peptide from Thosea asigna virus, codon-optimized for Drosophila melanogaster. | Causes co-translational cleavage of the encoded polypeptide. Multiple proteins can be made from a polycistronic transcript containing multiple ORFs separated by 2A. P2A and T2A have higher cleavage efficiency compared to other 2As. | 54 bp | View |
| P2A(dme) | Self-cleaving 2A peptide from Porcine teschovirus-1, codon-optimized for Drosophila melanogaster. | Causes co-translational cleavage of the encoded polypeptide. Multiple proteins can be made from a polycistronic transcript containing multiple ORFs separated by 2A. P2A and T2A have higher cleavage efficiency compared to other 2As. | 57 bp | View |
| E2A(dme) | Self-cleaving 2A peptide from Equine rhinitis A virus, codon-optimized for Drosophila melanogaster. | Causes co-translational cleavage of the encoded polypeptide. Multiple proteins can be made from a polycistronic transcript containing multiple ORFs separated by 2A. | 60 bp | View |
IRES（非連續(xù)翻譯）
| 姓名 | 描述 | Application Notes | Length | Details |
|---|---|---|---|---|
| IRES | Encephalomyocarditis virus internal ribosome entry site | Recruits ribosome to initiate translation internally on a transcript independent of its 5' end. Multiple proteins can be made from a polycistronic transcript containing multiple ORFs separated by IRES. | 588 bp | View |
| Ubx-IRES | Drosophila melanogaster Ultrabithorax internal ribosome entry site | Recruits ribosomes to initiate translation internally on a transcript independent of its 5' end. Multiple proteins can be made from a polycistronic transcript containing multiple ORFs separated by IRES. | 594 bp | View |
