元件指南

VectorBuilder提供了許多常用的載體元件，可供用戶在設(shè)計載體時進行選擇。下表按類型提供了這些常用元件的詳細信息。

姓名	靶序列	Target Gene	Target Position	Length	Details
Scramble[shRNA#1]	CCTAAGGTTAAGTCGCCCTCG	None in human and mouse		48 bp	View
Scramble[shRNA#2]	CAACAAGATGAAGAGCACCAA	TurboGFP; none in human and mouse		48 bp	View
EGFP[shRNA#1]	CGACGTAAACGGCCACAAGTT	EGFP	63-83	48 bp	View
EGFP[shRNA#2]	ACGTCTATATCATGGCCGACA	EGFP	449-469	48 bp	View
LacZ[shRNA#1]	GTCGGCTTACGGCGGTGATTT	LacZ	1734-1754	48 bp	View
LacZ[shRNA#2]	GTTCCGTCATAGCGATAACGA	LacZ	1908-1928	48 bp	View
Luciferase[shRNA#1]	ATGTTTACTACACTCGGATAT	Firefly luciferase	745-765	48 bp	View
Luciferase[shRNA#2]	TGTCCGGTTATGTAAACAATC	Firefly luciferase	1193-1213	48 bp	View
Scramble[miR30-shRNA#1]	ACCTAAGGTTAAGTCGCCCTCG	None in human and mouse		63 bp	View
Scramble[miR30-shRNA#2]	CCAACAAGATGAAGAGCACCAA	TurboGFP; none in human and mouse		63 bp	View
EGFP[miR30-shRNA#1]	GCGACGTAAACGGCCACAAGTT	EGFP	62-83	63 bp	View
EGFP[miR30-shRNA#2]	AACGTCTATATCATGGCCGACA	EGFP	448-469	63 bp	View
EGFP[miR30-shRNA#3]	AAGAACGGCATCAAGGTGAACT	EGFP	475-496	63 bp	View
EGFP[miR30-shRNA#4]	CACATGAAGCAGCACGACTTCT	EGFP	232-253	63 bp	View